CD51 (Integrin alpha V) Monoclonal Antibody (RMV-7), eBioscience™, Invitrogen™

Description

• Description: The RMV-7 monoclonal antibody reacts with the mouse CD51 molecule, the integrin alpha v chain
• This approximately 120 kDa surface molecule non-covalently associates with the beta subunits of the integrin family including beta3 (CD61), beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components
• Heterodimers of CD51/CD61 are expressed by platelets, T cells and granulocytes and mediate adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin
• Applications Reported: The RMV-7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and functional assays
• Soluble RMV-7 blocks adhesion to fibronectin and vitronectin and immobilized RMV-7 induces activation
• (Please use Functional Grade purified RMV-7, cat
• 16-0512, in functional assays.)
• Applications Tested: The RMV-7 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• ITAGV encodes integrin alpha chain V
• Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain
• The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins
• Among the known associating beta chains (beta chains 1,3,5,6, and 8, 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands, the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR)
• In addition to adhesion, many integrins are known to facilitate signal transduction
• The ITGB3 protein product is the integrin beta chain beta 3
• Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain
• A given chain may combine with multiple partners resulting in different integrins
• Integrin beta 3 is found along with the alpha IIb chain in platelets
• Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.