CD93 (AA4.1) Monoclonal Antibody (R139), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody R139 recognizes human CD93, also known as C1qRp
• The glycoprotein CD93 binds to C1q, the subunit of the complement protein, mannose binding lectin and pulmonary surfactant protein A
• CD93 is predicted to play a role in the clearance of apoptotic cells
• Expression of CD93 is confined to myeloid cells with higher expression on monocytes than neutrophils, eosinophils, platelets and endothelial cells
• Expression on DC's is downregulated upon maturation
• Additionally, CD93 has been shown to define an early bone marrow stem cell population of hematopoietic and hepatic precursors
• The monoclonal antibody R139 blocks C1q-mediated enhancement of phagocytosis
• CD93 can be shed from the cell surface
• This phenomenon can be measured using R3 antibody as detection with R139 as capture to detect soluble CD93 by ELISA
• The epitope for R139 resides in the EGF domains
• Applications Reported: This R139 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) under nonreducing conditions, ELISA, and immunohistochemistry of formalin-fixed paraffin embedded tissue
• Applications Tested: This R139 antibody has been tested by flow cytometric analysis of human peripheral blood cells
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker
• The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells
• The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.