CD100 (SEMA4D) Monoclonal Antibody (BMA12 (BMA-12)), eBioscience™, Invitrogen™

Description

• Description: The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D
• Sema4D/CD100 is a 150kDa transmembrane protein expressed constitutively on T cells and is involved in the activation of B cells through its interaction with CD72
• Interaction of these two proteins diminishes the negative signaling normally effected by CD72
• CD100 is already expressed strongly on resting T cells and somewhat weakly on B cells, but activation upregulates expression on both cell types
• Antigen expression has been reported in Spleen, thymus and lymph nodes
• Applications Reported: The BMA12 (BMA-12) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining
• It has also been reported for use in neutralization of mouse CD100
• (Please use Functional Grade purified BMA12, cat
• 16-1001, in functional assays.)
• Applications Tested: The BMA12 (BMA-12) antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development
• CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro
• CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression
• CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling
• CD100 is expressed on resting and PHA stimulated T cells
• The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.