CD180 (RP105) Monoclonal Antibody (MHR73-11), eBioscience™, Invitrogen™

Description

• Description: The MHR73-11 monoclonal antibody reacts with human CD180 (RP105)
• This 105 kDa type I transmembrane molecule is a member of the TLR family of proteins characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor
• RP105 physically associates with another molecule called MD-1 and is expressed on B, monocytes/macrophages, and dendritic cells
• Histological studies show that RP105 is expressed mainly on mature B cells in mantle zones, while germinal center cells are either dull or negative
• The RP105/MD-1 complex in concert with TLR4 mediates B cell recognition and signaling of LPS
• MHR73-11 activates B cells, leading to increases in cell size, expression of the costimulatory molecule CD80, and DNA synthesis
• Moreover, ligation of RP105 protects B cells from irradiation- or dexamethasone-induced apoptosis
• Thus, RP105 is a signal transduction molecule and plays a role in regulation of B cell growth and death
• A significant proportion of circulating B cells in SLE patients is RP105 negative
• Loss of RP105 is associated with B cell activation and increased disease activity in SLE patients
• Applications Reported: MHR73-11 has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining
• MHR73-11 has also been reported in in vitro functional studies
• (Please use Functional Grade purified MHR73-11, cat
• 16-1809, in functional assays.)
• CD180 (RP105, Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family
• CD180 cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain
• CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells
• CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands
• Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size
• CD180 consists of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail
• The extracellular LRR is part of the cell surface receptor complex, RP105/MD-1
• Diseases associated with CD180 dysfunction include Kawasaki Disease in children and may play a role in rheumatic disease pathology.