CD140b (PDGFRB) Monoclonal Antibody (APB5), eBioscience™, Invitrogen™

Description

• Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor)
• PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates
• Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity
• CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues
• The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions
• Applications Reported: The APB5 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections
• It has also been reported in blocking of ligand binding
• (Please use Functional Grade purified APB5, cat
• 16-1402, in functional assays.)
• Applications Tested: The APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• PDGFRB is a receptor tyrosine kinase and has been implicated in atherogenesis and in cell transformation by the SIS oncogene
• These growth factors are mitogens for cells of mesenchymal origin
• The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides
• The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome
• A translocation between chromosomes 5 and 12, that fuses PDGFRB (CD140b) to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.