Nod2 Monoclonal Antibody (26mNOD2), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 26mNOD2 reacts with mouse NOD2
• NOD2, also referred to as caspase recruitment domain-containing protein 15 (CARD 15), is a cytosolic protein of approximately 115kDa
• NOD2 contains a central NOD (nucleotide oligomerization domain) that is flanked by a C-terminal leucine-rich repeat (LRR) domain and an N-terminal CARD domain
• The LRR confers recognition of microbial components such as muramyl dipeptide and the CARD domain confers the ability to interact with RIP2 via homotypic interaction with the RIP2 CARD
• This interaction leads to downstream activation of NF-kappaB
• The antibody 26mNOD2 has been shown to recognize protein from transgenic and transfected cells
• Refer to references for expression in endogenous cells
• Applications Reported: This 26mNOD2 antibody has been reported for use in immunoblotting (WB)
• Applications Tested: This 26mNOD2 antibody has been tested by immunoblotting of lysates of 293T cells transfected with mouse NOD2
• This antibody has been validated on transfected cell lines and cells from NOD2-transgenic mice
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• NOD2 is a member of the apoptosis regulating protein family that includes caspase recruitment-domains, as well as Apaf-1 and NOD1
• It contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs)
• NOD2 is expressed in monocytes (whereas NOD1 is expressed in multiple tissues)
• NOD2 plays a role in regulating NF-kappaB, acts as an intracellular receptor for bacterial lipopolysaccharides, and contributes to inflammatory bowel disease (IBD) and Crohn's disease.