CD321 (F11R) Monoclonal Antibody (WK9), eBioscience™, Invitrogen™

Description

• Description: CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors
• CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail
• The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins
• CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, epithelial and endothelial cells, and plays a role in mediating cell-cell contact
• CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin
• CD321 is also involved in reovirus entry into cells
• Applications Reported: This WK9 antibody has been reported for use in flow cytometric analysis and immunoprecipitation
• The WK9 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 38 kD under reducing and non-reducing conditions
• Applications Tested: This WK9 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors
• CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail
• The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins
• CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, stem cells, epithelial and endothelial cells, and plays a role in mediating cell-cell contact
• CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin
• CD321 is involved in the maintenance of epithelial and endothelial barriers, hematopoiesis, angiogenesis, and tumor proliferation, invasion, and metastasis.