Caspase 1 Monoclonal Antibody (5B10), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 5B10 recognizes mouse Caspase-1, a member of the cysteine-requiring aspartate protease family, which plays a critical role in cell suicide known as apoptosis
• In particular, it converts pro-IL-1beta to mature IL-1beta; hence it is also called ICE (interleukin-1beta converting enzyme)
• Upon activation Caspase-1 exists as a tetramer of two p20 and two p10 subunits
• There are several isoforms of Caspase-1
• The 5B10 antibody recognizes both the pro-form of about 42kDa and the active p20 form
• Applications Reported: This 5B10 antibody has been reported for use in immunoblotting (WB)
• Applications Tested: This 5B10 antibody has been tested by western blot analysis of lysates prepared from the J774 cell line
• This can be used at 1-5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies
• Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens
• Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18
• Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C
• elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.