SAP (SLAM-Associated Protein) Monoclonal Antibody (10C4.2), eBioscience™, Invitrogen™

Description

• Description: This 10C4.2 monoclonal antibody reacts with human SLAM-associated protein (SAP)
• Upon SLAM receptor ligation, this 16-kDa SH2 domain-containing adaptor protein recruits Fyn tyrosine kinase, which phosphorylates the receptor to activate downstream signaling events
• SAP is expressed in T, NK, and NKT cells, as well as eosinophils and platelets
• This protein has also been detected in B cells and tumor cell lines such as Jurkat and Raji
• Studies demonstrate that SAP expression in T follicular helper cells is required for germinal center formation
• Moreover, this protein is involved in NKT cell development, Th1/Th2 differentiation, and controlling NK cytotoxicity
• The role of SAP in B cells, however, is currently under investigation
• Finally, deletion of the gene encoding SAP, SH2D1A, results in X-linked lymphoproliferative disease in humans
• Applications Reported: This 10C4-SAP (10C4.2) antibody has been reported for use in immunoblotting and immunocytochemistry (ICC)
• Applications Tested: This 10C4.2 antibody has been tested by immunoblotting of reduced cell lysate prepared from Jurkat cells
• This can be used at less than or equal to 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells
• This protein contains an SH2 domain and a short tail
• It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site
• This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells
• Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma
• Multiple transcript variants encoding different isoforms have been found for this gene.