50-138-23
Nestin Monoclonal Antibody (10C2), eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-138-23-Each-of-1 | In Stock | ₹ 42,780.00 |
50-138-23 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,780.00
GST (18%): ₹ 7,700.40
Total Price: ₹ 50,480.40
Antigen
Nestin
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P48681
Gene Symbols
Nes
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
10763
Content And Storage
4° C
Form
Liquid
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Clone
10C2
Conjugate
Unconjugated
Gene
Nes
Gene Alias
AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1
Description
- Description: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614)
- Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system
- Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential
- Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP
- Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors
- No cross-reactivity to rodent nestins has been observed using the 10C2 antibody
- Applications Reported: This 10C2 antibody has been reported for use in immunocytochemistry, microscopy, western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and flow cytometric analysis
- Applications Tested: This 10C2 antibody has been tested by western blot on SDS-reduced and non-reduced lysates from U251 cells and can be used at less than or equal to 5 μg/mL
- The 10C2 antibody has also been tested by immunocytochemistry on formaldehyde-fixed and permeabilized SKNSH cells and can be used at less than or equal to 5 μg/mL
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells
- Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells
- Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors
- Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells
- Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
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Antigen: Nestin
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: P48681
Gene Symbols: Nes
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 10763
Content And Storage: 4° C
Form: Liquid
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Clone: 10C2
Conjugate: Unconjugated
Gene: Nes
Gene Alias: AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human
Product Type: Antibody
Isotype: IgG1
Antigen:
Nestin
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
P48681
Gene Symbols:
Nes
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
10763
Content And Storage:
4° C
Form:
Liquid
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Clone:
10C2
Conjugate:
Unconjugated
Gene:
Nes
Gene Alias:
AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human
Product Type:
Antibody
Isotype:
IgG1
Antigen: SAP (SLAM-Associated Protein)
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: O60880
Gene Symbols: Sh2d1a
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 4068
Content And Storage: 4° C
Form: Liquid
Applications: Immunocytochemistry, Western Blot
Clone: 10C4.2
Conjugate: Unconjugated
Gene: Sh2d1a
Gene Alias: DSHP; Duncan disease homolog; Duncan disease SH2-protein; Duncan's disease; EBVS; FLJ18687; FLJ92177; Gm686; HGNC:10820; IMD5; LYP; MTCP1; RGD1562408; RP5-1052M9.3; SAP; SAP/SH2D1A; SH2 domain containing 1A; SH2 domain protein 1A; SH2 domain-containing protein 1A; SH2D1A; signaling lymphocyte activation molecule-associated protein; signaling lymphocytic activation molecule-associated protein; SLAM associated protein/SH2 domain protein 1A; SLAM-associated protein; SLAM-associated protein isoform SAP-2; T cell signal transduction molecule SAP; T-cell signal transduction molecule SAP; Xlp; XLPD; XLPD1
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Human
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
SAP (SLAM-Associated Protein)
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
O60880
Gene Symbols:
Sh2d1a
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
4068
Content And Storage:
4° C
Form:
Liquid
Applications:
Immunocytochemistry, Western Blot
Clone:
10C4.2
Conjugate:
Unconjugated
Gene:
Sh2d1a
Gene Alias:
DSHP; Duncan disease homolog; Duncan disease SH2-protein; Duncan's disease; EBVS; FLJ18687; FLJ92177; Gm686; HGNC:10820; IMD5; LYP; MTCP1; RGD1562408; RP5-1052M9.3; SAP; SAP/SH2D1A; SH2 domain containing 1A; SH2 domain protein 1A; SH2 domain-containing protein 1A; SH2D1A; signaling lymphocyte activation molecule-associated protein; signaling lymphocytic activation molecule-associated protein; SLAM associated protein/SH2 domain protein 1A; SLAM-associated protein; SLAM-associated protein isoform SAP-2; T cell signal transduction molecule SAP; T-cell signal transduction molecule SAP; Xlp; XLPD; XLPD1
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Human
Product Type:
Antibody
Isotype:
IgG1 κ
Antigen: GFAP
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: P03995, P14136, P47819
Gene Symbols: GFAP
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 100352532, 14580, 24387, 2670, 396562, 419969
Content And Storage: 4° C
Form: Liquid
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone: GA5
Conjugate: Unconjugated
Gene: GFAP
Gene Alias: 6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
Host Species: Mouse
Quantity: 25 μg
Primary or Secondary: Primary
Target Species: Chicken, Human, Mouse, Porcine, Rabbit, Rat
Product Type: Antibody
Isotype: IgG1
Antigen:
GFAP
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
P03995, P14136, P47819
Gene Symbols:
GFAP
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
100352532, 14580, 24387, 2670, 396562, 419969
Content And Storage:
4° C
Form:
Liquid
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone:
GA5
Conjugate:
Unconjugated
Gene:
GFAP
Gene Alias:
6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
Host Species:
Mouse
Quantity:
25 μg
Primary or Secondary:
Primary
Target Species:
Chicken, Human, Mouse, Porcine, Rabbit, Rat
Product Type:
Antibody
Isotype:
IgG1
Antigen: GFAP
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: P03995, P14136, P47819
Gene Symbols: GFAP
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 100352532, 14580, 24387, 2670, 396562, 419969
Content And Storage: 4° C
Form: Liquid
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone: GA5
Conjugate: Unconjugated
Gene: GFAP
Gene Alias: 6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Chicken, Human, Mouse, Porcine, Rabbit, Rat
Product Type: Antibody
Isotype: IgG1
Antigen:
GFAP
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
P03995, P14136, P47819
Gene Symbols:
GFAP
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
100352532, 14580, 24387, 2670, 396562, 419969
Content And Storage:
4° C
Form:
Liquid
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone:
GA5
Conjugate:
Unconjugated
Gene:
GFAP
Gene Alias:
6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Chicken, Human, Mouse, Porcine, Rabbit, Rat
Product Type:
Antibody
Isotype:
IgG1