50-140-75

CD80 (B7-1) Monoclonal Antibody (16-10A1), PE-Cyanine5, eBioscience™, Invitrogen™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 50-140-75-Each-of-1 In Stock ₹ 29,946.00

50-140-75 - Each of 1

₹ 29,946.00

In Stock

Quantity

1

Base Price: ₹ 29,946.00

GST (18%): ₹ 5,390.28

Total Price: ₹ 35,336.28

Antigen

CD80 (B7-1)

Classification

Monoclonal

Concentration

0.2 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

Q00609

Gene Symbols

CD80

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

12519, 397161, 403765

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

16-10A1

Conjugate

PE-Cyanine5

Gene

CD80

Gene Alias

Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1

Host Species

Armenian Hamster

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Canine, Mouse, Porcine

Product Type

Antibody

Isotype

IgG

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Description

  • Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily
  • CD80 is expressed by macrophages, dendritic cells and activated B cells
  • In addition, activated T cells express this antigen
  • CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4)
  • The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively
  • Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes
  • This can be used at less than or equal to 0.06 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Light sensitivity: This tandem dye is sensitive photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
  • CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152)
  • Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process
  • CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes
  • The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells
  • Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

Compare Similar Items

Show Difference

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Fischer Scientific

50-140-75

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Antigen:
CD80 (B7-1)

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
Q00609

Gene Symbols:
CD80

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
12519, 397161, 403765

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
16-10A1

Conjugate:
PE-Cyanine5

Gene:
CD80

Gene Alias:
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1

Host Species:
Armenian Hamster

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Canine, Mouse, Porcine

Product Type:
Antibody

Isotype:
IgG

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Antigen:
CD90.1 (Thy-1.1)

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P01830, P01831

Gene Symbols:
Thy1

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
21838, 24832

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
HIS51

Conjugate:
PE-Cyanine5

Gene:
Thy1

Gene Alias:
CD7; CD90; FLJ33325; T25; Thy 1.2; Thy1; Thy-1; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 glycoprotein; thy-1 membrane glycoprotein; Thy-1 protein; Thy1.1; Thy1.2; Thy-1.2; thymus cell antigen 1, theta; Thymus cell surface antigen

Host Species:
Mouse

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse, Rat

Product Type:
Antibody

Isotype:
IgG2a κ

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Antigen:
CD90.2 (Thy-1.2)

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P01831

Gene Symbols:
Thy1

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
21838

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
53-2.1

Conjugate:
PE-Cyanine5

Gene:
Thy1

Gene Alias:
CD90; FLJ33325; T25; Thy 1.2; Thy1; Thy-1; Thy-1 antigen; thy-1 membrane glycoprotein; Thy1.1; Thy1.2; Thy-1.2; thymus cell antigen 1, theta

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a κ

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