7090372

CD80 (B7-1) Monoclonal Antibody (16-10A1), PE-Cyanine7, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD80 (B7-1)

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1

Gene Symbols

CD80

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

CD80

Clone

16-10A1

Applications

Flow Cytometry

Conjugate

PE-Cyanine7

Host Species

Armenian Hamster

Target Species

Canine, Mouse, Porcine

Gene Accession No.

Q00609

Gene ID (Entrez)

12519, 397161, 403765

Isotype

IgG

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily
  • CD80 is expressed by macrophages, dendritic cells and activated B cells
  • In addition, activated T cells express this antigen
  • CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4)
  • The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively
  • Applications Reported: This 16-10A1 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 16-10A1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes
  • This can be used at less than or equal to 1 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (cat
  • 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
  • CD80 (B7-1) Monoclonal antibody specifically detects CD80 (B7-1) in Canine, Mouse, Porcine samples
  • It is validated for Flow Cytometry.

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Antigen:
CD80 (B7-1)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1

Gene Symbols:
CD80

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD80

Clone:
16-10A1

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Armenian Hamster

Target Species:
Canine, Mouse, Porcine

Gene Accession No.:
Q00609

Gene ID (Entrez):
12519, 397161, 403765

Isotype:
IgG

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Concentration:
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Classification:
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Form:
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Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
activation B7-2 antigen; B lymphocyte activation antigen B72; B7; B7.2; B70; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86; CD86 antigen; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 antigen precursor; CD86 molecule; CLS1; CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; early T-cell costimulatory molecule 1; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly58; Ly-58; MB7; MB7-2; membrane glycoprotein; MGC34413; T-lymphocyte activation antigen CD86; TS/A-2

Gene Symbols:
CD86

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD86

Clone:
GL1

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P42082

Gene ID (Entrez):
12524

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Concentration:
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Classification:
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Form:
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Regulatory Status:
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Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
CD90; FLJ33325; T25; Thy 1.2; Thy1; Thy-1; Thy-1 antigen; thy-1 membrane glycoprotein; Thy1.1; Thy1.2; Thy-1.2; thymus cell antigen 1, theta

Gene Symbols:
Thy1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Thy1

Clone:
53-2.1

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P01831

Gene ID (Entrez):
21838

Isotype:
IgG2a κ

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Antigen:
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Concentration:
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Classification:
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Form:
Liquid

Regulatory Status:
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Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Cd101; CD101 antigen; CD101 molecule; cell surface glycoprotein V7; EWI immunoglobulin subfamily member EWI-101; EWI101; EWI-101; Glu-Trp-Ile EWI motif-containing protein 101; Gm1016; Gm734; IGSF2; Immunoglobulin superfamily member 2; immunoglobulin superfamily, member 2; leukocyte surface protein; RP11-27K13.2; V7

Gene Symbols:
CD101

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD101

Clone:
Moushi101

Applications:
Flow Cytometry

Conjugate:
PE-Cyanine7

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
A8E0Y8

Gene ID (Entrez):
630146

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
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