CD80 (B7-1) Monoclonal Antibody (16-10A1), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily
• CD80 is expressed by macrophages, dendritic cells and activated B cells
• In addition, activated T cells express this antigen
• CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4)
• The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively
• Applications Reported: This 16-10A1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 16-10A1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152)
• Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process
• CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes
• The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells
• Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.