Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6)
• Mouse Ly108 is also known as NTB-A (NK cell, T cell, B cell antigen), and is expressed on NK, T cells, and B cells
• Engagement of the Ly108 receptor enhances NK cell activity and expression of IFN-gamma, and contributes to the inability of NK cells to kill Epstein Barr virus-infected B cells in X-linked lymphoproliferative disease
• CD4+ T cells from Ly108 knock-out mice exhibit diminished ability to produce IL-4 and defective neutrophil functions
• Staining has not revealed any strain differences between C57BL/6 and BALB/c mice
• Other strains have not been tested
• Applications Reported: This eBio13G3-19D (13G3-19D) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB)
• Applications Tested: This eBio13G3-19D (13G3-19D) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Endotoxin: Less than 0
• Ly108 is the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6)
• Mouse Ly108 is expressed on NK, B cells, and T cells
• Activation of Ly108 receptor enhances NK cell activity and IFN-gamma expression.