Ly-49H Monoclonal Antibody (3D10), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells
• Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors
• Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells
• Natural killer cells can express several Ly-49 proteins
• It has been shown that there is preferential staining of Ly-49H with Ly-49D (cat 12-5783)
• Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice
• Other mice strains have not been tested
• No crossreactivity to Ly-49A, C, D or G2 has been observed
• Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells
• Applications Reported: This 3D10 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation
• Applications Tested: This 3D10 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells
• Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors
• Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells
• Natural killer cells can express several Ly49 proteins.