Ly-49E/F Monoclonal Antibody (CM4), APC, eBioscience™, Invitrogen™

Description

• Description: This CM4 monoclonal antibody reacts with a subset of mouse Ly49 receptors, specifically Ly49E and Ly49F
• The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6
• Expression is confined to NK cell, lymphocytes that play a key role in protection from infection and maligt diseases as well as graft rejection and autoimmune diseases
• Ly49E is unique from other family members in that it contains an ITIM (immunoreceptor tyrosine-based inhibitory motif) domain and is expressed on all mouse strains
• Developmentally Ly49E is one of the first markers detected on NK cells but expression decreases after birth to about 1% of NK cells in the adult spleen
• Upon stimulation, expression can again be induced on Ly49E negative cells
• The CM4 antibody is a useful reagent for the characterization of Ly49E expression when used in combination with other Ly49 antibodies such as 14B11 (Ly49C/I/F/H), 3D10 (Ly49C), YLI-90 (Ly49I), and A1 (Ly49A)
• Applications Reported: This CM4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This CM4 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• The Ly49 family of receptors are clustered in the Natural Killer Cell complex on chromosome 6
• Expression is confined to NK cell, lymphocytes that play a key role in protection from infection and malignant diseases as well as graft rejection and autoimmune diseases
• Ly49E is unique from other family members in that it contains an ITIM (immunoreceptor tyrosine-based inhibitory motif) domain and is expressed on all mouse strains
• Developmentally Ly49E is one of the first markers detected on NK cells but expression decreases after birth to about 1% of NK cells in the adult spleen
• Upon stimulation, expression can again be induced on Ly49E negative cells
• The CM4 antibody is a useful reagent for the characterization of Ly49E expression when used in combination with other Ly49 antibodies such as 14B11 (Ly49C/I/F/H), 3D10 (Ly49C), YLI-90 (Ly49I), and A1 (Ly49A).