KLRG1 Monoclonal Antibody (2F1), Pacific Orange™, eBioscience™, Invitrogen™

Description

• This 2F1 MAb reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA)
• KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM)
• Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells
• This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells
• MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling
• Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct
• KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains
• These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1
• KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells
• NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.