DFF45/DFFA Polyclonal Antibody, Bethyl Laboratories

Description

• The recommended shelf life for this product is 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
• ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis
• ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35
• Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional
• ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation
• In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD
• The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD
• In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone
• There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells
• Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.