CD117 (c-Kit) Monoclonal Antibody (104D2), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The 104D2 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor
• A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells
• The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation
• CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells
• Applications Reported: This 104D2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 104D2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Please make sure that your instrument is capable of detecting this fluorochrome
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor)
• KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit
• KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells
• Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism
• Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth
• c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells
• Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor
• This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway
• SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT
• c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.