CD90.1 (Thy-1.1) Monoclonal Antibody (HIS51), APC-eFluor™ 780, eBioscience™, Invitrogen™

Description

• Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule
• In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types
• In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells
• Applications Reported: This HIS51 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HIS51 antibody has been tested by flow cytometric analysis of rat splenocytes
• This can be used at less than or equal to 0.06 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Light sensitivity: This tandem is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family
• It is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts
• Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.