CD105 (Endoglin) Monoclonal Antibody (MJ7/18), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin
• This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells
• It is suggested that CD105 functions in adhesion and embryonic angiogenesis
• Applications Reported: This MJ7/18 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MJ7/18 antibody has been tested by flow cytometric analysis of bEnd.3 cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells
• It exists on an O- and N-glycosylated homodimer
• Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker
• CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.