CD45 Monoclonal Antibody (YKIX716.13), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: This YKIX716.13 monoclonal antibody reacts with canine CD45, also known as the leukocyte common antigen
• CD45 is expressed by all hematopoietic cells except red blood cells and platelets
• CD45 exists as multiple isoforms due to alternative splicing
• The C-terminal domain of CD45 exhibits tyrosine phosphatase activity that enables signaling by T cell and B cell receptors following activation
• This monoclonal antibody recognizes all isoforms of CD45
• In addition, when used in conjunction with YKIX753.22, this antibody has been reported to promote cell depletion via antibody-dependent cell-mediated cytotoxicity
• Applications Reported: This YKIX716.13 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This YKIX716.13 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
• CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues
• CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
• CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains
• CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
• CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
• CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling
• CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.