CD357 (AITR/GITR) Monoclonal Antibody (eBioAITR), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody reacts with human AITR, Activation Inducible TNFR family member, an approximately 25 kDa member of the TNFR superfamily
• AITR mRNA is detected in lymph node, peripheral blood leukocytes and weakly in spleen
• AITR mRNA expression was upregulated within 24 hour on PMA/ionomycin or PHA stimulated PBMC
• At the protein level, AITR is expressed by a small population of activated PBMC
• AITR associates with TRAF1, TRAF2 and TRAF3 and induces nuclear factor NF-kappaB activation via TRAF2
• Recently TL6 (AITRL) has been reported as the ligand for AITR
• Interaction of AITR with AITRL is important for cross-talk between T lymphocytes and endothelial cells
• Applications Reported: This eBioAITR antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioAITR antibody has been pre-titrated and tested by flow cytometric analysis of PHA-stimulated normal peripheral blood mononuclear cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene
• It is mapped to 1p36.33
• This gene encodes a member of the TNF-receptor superfamily
• The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells
• Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death
• Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.