CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), Super Bright™ 702, eBioscience™, Invitrogen™

Description

• Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18
• In naive mice, GITR is expressed predomitly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes
• Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression
• Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis
• The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease
• Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.03 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm
• We recommend using a 710/50 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene
• It is mapped to 1p36.33
• This gene encodes a member of the TNF-receptor superfamily
• The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells
• Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death
• Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.