50-168-85

CD33 Monoclonal Antibody (WM-53 (WM53)), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each of 1 50-168-85-Each-of-1 In Stock ₹ 33,197.28

50-168-85 - Each of 1

₹ 33,197.28

In Stock

Quantity

1

Base Price: ₹ 33,197.28

GST (18%): ₹ 5,975.51

Total Price: ₹ 39,172.79

Antigen

CD33

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

P20138

Gene Symbols

CD33

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

945

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

WM-53 (WM53)

Conjugate

Alexa Fluor 700

Gene

CD33

Gene Alias

CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

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Description

  • Description: The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family
  • It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells
  • Applications Reported: This WM-53 (WM53) antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This WM-53 (WM53) antibody has been pre-titrated and tested by flow cytometric analysis of lysed whole blood
  • This can be used at 5 μL (0.5 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Alexa Fluor™ 700 emits at 723 nm and can be excited with the red laser (633 nm)
  • Most instruments will require a 685 LP mirror and 710/20 filter
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family
  • It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface
  • CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia
  • Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells
  • CD33 is also expressed on, and is a useful marker for, peripheral monocytes
  • CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias
  • Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

Compare Similar Items

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Invitrogen

50-168-85

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Antigen:
CD33

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P20138

Gene Symbols:
CD33

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
945

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
WM-53 (WM53)

Conjugate:
Alexa Fluor 700

Gene:
CD33

Gene Alias:
CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ

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