62033942

CD33 Monoclonal Antibody (HIM3-4), Super Bright™ 436, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each of 1 62033942-Each-of-1 In Stock ₹ 31,742.76

62033942 - Each of 1

₹ 31,742.76

In Stock

Quantity

1

Base Price: ₹ 31,742.76

GST (18%): ₹ 5,713.697

Total Price: ₹ 37,456.457

Antigen

CD33

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

P20138

Gene Symbols

CD33

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

945

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

HIM3-4

Conjugate

Super Bright 436

Gene

CD33

Gene Alias

CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

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Description

  • Description: The HIM3-4 monoclonal antibody reacts with human CD33, a 67 kDa member of the sialoadhesion family
  • CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes
  • Hematopoietic stem cells and lymphocytes do not express this antigen
  • CD33 plays a role in sialic-acid dependent cell adhesion
  • Applications Reported: This HIM3-4 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This HIM3-4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
  • This may be used at 5 μL (0.25 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
  • We recommend using a 450/50 bandpass filter, or equivalent
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
  • SB-4400) to minimize any non-specific polymer interactions
  • Please refer to the datasheet for Super Bright Staining Buffer for more information
  • Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family
  • It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface
  • CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia
  • Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells
  • CD33 is also expressed on, and is a useful marker for, peripheral monocytes
  • CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias
  • Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

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Antigen:
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Classification:
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Concentration:
5 μL/Test

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P20138

Gene Symbols:
CD33

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
945

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
HIM3-4

Conjugate:
Super Bright 436

Gene:
CD33

Gene Alias:
CD33; CD33 antigen; CD33 antigen (gp67); CD33 molecule; CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC3; Siglec-3

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ

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__

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__

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__

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__

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Classification:
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Concentration:
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PBS with BSA and 0.09% sodium azide

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Gene Symbols:
ENTPD1

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

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953

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
eBioA1 (A1)

Conjugate:
Super Bright 436

Gene:
ENTPD1

Gene Alias:
2610206B08Rik; AA408691; ATPDase; Cd39; CD39 antigen; DKFZp686D194; DKFZp686I093; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; ecto-ATPase 1; ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1; NTPDase-1; SPG64

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PBS with BSA and 0.09% sodium azide; pH 7.2

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Q01151

Gene Symbols:
CD83

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
9308

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
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Clone:
HB15e

Conjugate:
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Gene:
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Gene Alias:
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