CD54 (ICAM-1) Monoclonal Antibody (R6.1), eBioscience™, Invitrogen™

Description

• Description: Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-betacomplex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors
• ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55 kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B cells and dendritic cells in tonsils, lymph nodes and Peyer's patches
• ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions
• ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR
• sICAM-1 serum levels were found elevated in acute renal graft rejection, insulin-dependent diabetis mellitus, anterior uveitis and in allergic inflammation
• Applications Tested: ELISA
• ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain
• ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102)
• ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor
• ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation
• ICAM-1 is also detected in some carcinoma and melanoma cells
• Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.