56-054-941

CD54 (ICAM-1) Monoclonal Antibody (HA58), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each of 1 56-054-941-Each-of-1 In Stock ₹ 13,090.68

56-054-941 - Each of 1

₹ 13,090.68

In Stock

Quantity

1

Base Price: ₹ 13,090.68

GST (18%): ₹ 2,356.322

Total Price: ₹ 15,447.002

Antigen

CD54 (ICAM-1)

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

P05362

Gene Symbols

ICAM1

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

3383

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

HA58

Conjugate

Alexa Fluor 700

Gene

ICAM1

Gene Alias

BB2; CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM; Icam1; ICAM-1; ICAM-1; CD54 homolog; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; intercellular adhesion molecule-1 precursor; Ly 47; Ly-47; major group rhinovirus receptor; MALA2; MALA-2; MyD10; P3.58; sCD54; soluble CD54; Surface antigen of activated B cells

Host Species

Mouse

Quantity

25 Tests

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

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Description

  • Description: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells
  • Expression of CD54 is upregulated on activated lymphocytes
  • Interaction of CD54 with its ligand CD11a is important in the inflammatory response
  • Applications Reported: This HA58 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This HA58 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
  • This may be used at 5 μL (0.5 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
  • Most instruments will require a 685 LP mirror and 710/20 band pass filter
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain
  • ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102)
  • ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor
  • ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation
  • ICAM-1 is also detected in some carcinoma and melanoma cells
  • Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

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Antigen:
CD54 (ICAM-1)

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
P05362

Gene Symbols:
ICAM1

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
3383

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
HA58

Conjugate:
Alexa Fluor 700

Gene:
ICAM1

Gene Alias:
BB2; CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM; Icam1; ICAM-1; ICAM-1; CD54 homolog; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; intercellular adhesion molecule-1 precursor; Ly 47; Ly-47; major group rhinovirus receptor; MALA2; MALA-2; MyD10; P3.58; sCD54; soluble CD54; Surface antigen of activated B cells

Host Species:
Mouse

Quantity:
25 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ

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PBS with 0.5% BSA, 10% proprietary stabilizer and 0.05% sodium azide; pH 7.2

Gene Accession No.:
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Gene Symbols:
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Purification Method:
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Primary or Secondary:
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Human

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