CD56 (NCAM) Monoclonal Antibody (TULY56), Super Bright™ 702, eBioscience™, Invitrogen™

Description

• Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family
• CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1
• These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues
• Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.03 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm
• We recommend using a 710/50 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
• CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa
• CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity
• Polysialic modification results in reduction of CD56-mediated cell adhesion
• Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins
• CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma
• Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors
• Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.