LIF Monoclonal Antibody (1F10), eBioscience™, Invitrogen™

Description

• Description: The 1F10 antibody specifically binds to human Leukemia Inhibitory Factor a cytokine which was primarily described as a product of activated T lymphocytes and macrophages
• It is a variably glycosylated 180 amino acid protein of apparent molecular weight between 35 and 67 kDa depending on its source and glycosylation
• The human LIF-gene is located on chromosome 22, the gene product is highly conserved between species
• The multivarious nature of LIF is illustrated by its multiple discoveries under a variety of synonyms
• LIF exerts its pleiotropic functions in a wide variety of systems
• Examples for its important and diverse physiological functions are support of growth and inhibition of differentiation of normal embryonic stem cells, stimulation of the production of acute-phase proteins by hepatocytes
• Applications Tested: ELISA, non-blocking
• Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family
• It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family
• LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue
• LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis
• LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity
• However, LIF inhibition of stem cell differentiation appears to be mouse-specific.