IL-12 p35 Monoclonal Antibody (B-T21 (BT21)), eBioscience™, Invitrogen™

Description

• Description: BMS152 (B-T21) recognizes natural and recombinant human IL-12A p35/p70 but not IL-12-p40
• BMS 152 blocks IL-12 induced proliferation on PHA and IL-2 activated lymphoblasts
• Biologically active IL-12 is a disulfide-linked heterodimeric 70-kDa cytokine composed of a 35-kDa (p35) and a 40-kDa (p40) subunit
• p35, a member of the IL-6 superfamily, is secreted in response to IFN gamma and agonists of TLR3, 4, or 7
• However, p35 expression has been shown to be inhibited by Th2 cytokines and expressed at much lower levels than p40
• Expression of p40 is regulated independently of p35
• Moreover, p40 has been shown to be secreted as either a monomer or homodimer
• Although each subunit alone does not possess IL-12 bioactivity, the p40 homodimer can bind to the IL-12 receptor and act as an antagonist to IL-12 p70
• The receptor for this cytokine is composed of two subunits, IL-12R beta 1 and IL-12R beta 2, the latter of which is the signaling component of the receptor
• Applications Tested: Functional Studies (Blocking)
• Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily
• Interleukin-12 alpha chain precursor (IL-12A) is a 35-kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities
• Reports suggest that IL-12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK/lymphokine-activated Killer cells
• IL-12A stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells
• It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein
• The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A
• Along with EBI3, IL-12A forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.