IL-4 Monoclonal Antibody (OX81), eFluor™ 660, eBioscience™, Invitrogen™

Description

• This OX81 MAb reacts with rat interleukin (IL)-4
• Also known as BCDF, BCGF-1 and BSF-1, IL-4 is secreted by activated Th2 and natural killer T cells
• This cytokine is also produced by Th1 and mast cells albeit to a lesser extent
• IL-4 is species-specific and promotes proliferation and differentiation of B cells
• IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of MHC class II and CD23
• The OX81 antibody has been reported to exhibit neutralizing activity
• Applications Reported: This OX81 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This OX81 antibody has been tested by intracellular flow cytometric analysis of activated rat splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor 660 is a replacement for Alexa Fluor 647
• eFluor 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor
• IL-4 has been found to have the broadest range of actions
• The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13
• STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4
• The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q
• In particular, IL-4 is located in close proximity to IL13 on the chromosome
• Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome
• IL-4 delta 2 is a known splice variant of IL-4.