IL-2 Monoclonal Antibody (AB12-3G4), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: This AB12-3G4 monoclonal antibody reacts with and inhibits the bioactivity of human IL-2
• IL-2 is a 15 kDa cytokine expressed by B cells, T cells, and dendritic cells
• IL-2 signals via a heterotrimeric receptor (CD25) and results in the activation of Jak/STAT pathways
• IL-2 plays a critical role in the proliferation, activation, and differentiation of T cells
• The development of CD4+CD25+ regulatory T cells is especially dependent on the presence of IL-2, making it an important immunoregulatory cytokine
• Applications Reported: This AB12-3G4 monoclonal antibody inhibits the bioactivity of human IL-2
• Applications Tested: The ND50 of AB12-3G4, as determined by the neutralization of Human IL-2-induced proliferation of the CTLL-2 cell line, is less than or equal to 60 ng/mL in the presence of 10 ng/mL Human IL-2 Recombinant Protein
• The neutralization dose of this antibody will vary depending on cell type, assay method, and cytokine concentration
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages
• IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7)
• The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene
• The targeted disruption of a gene similar to IL-2 in mice leads to an μgcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.