Bcl-2 Monoclonal Antibody (4D7), eBioscience™, Invitrogen™

Description

• Description: Bcl-2 is a key regulator of apoptosis and the founding member of Bcl-2 family of apoptosis regulatory protiens
• The anti-apoptotic functions of Bcl-2 is achieved by activation or inactivation of an inner mitochondrial permeability transition pore, which is involved in the regulation of matrix Ca2+, pH, and voltage
• Applications Tested: ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blotting
• BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death
• The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis
• Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2
• Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis
• Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells
• Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma
• In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative
• Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends
• The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.