CD18 (LFA-1 beta) Monoclonal Antibody (R3.3), eBioscience™, Invitrogen™

Description

• Description: BMS103 is also suitable as primary antibody in staining for FACS analysis, BMS103FI can be used for direct staining of cells, BMS103BT can be used in a 2-step procedure using the biotin-(strept)avidin system
• BMS103 can be used as a neutralizing agent
• Recommended CD18 is a 90-95 kDa type I transmembrane protein that heterodimerizes with the a chain of CD11a, CD11b and CD11c
• The CD18/CD11a complex is also known as lymphocyte function-associated antigen-1 (LFA-1) and plays an important role in cellular adhesion and inflammatory reactions
• CD18 is expressed broadly by human lymphocytes and monocytes
• Peripheral blood granulocytes also express CD18, but at lower levels
• Applications Tested: Flow Cytometry, Functional Studies (Blocking), Immunohistochemistry
• Purity: >95%
• CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes
• CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling
• CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18)
• In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts
• Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease
• CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis
• Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1)
• Two transcript variants encoding the CD18 protein have been identified.