CD95 (APO-1/Fas) Monoclonal Antibody (SM1/23), FITC, eBioscience™, Invitrogen™

Description

• Description: BMS140 is suitable as primary antibody for staining in FACS analysis
• BMS140FI can be used for direct staining of cells
• BMS140BT can be used in a two-step procedure using the biotin-(strept-)avidin system
• Functional studies: BMS140 was found to block induction of apoptosis induced by clone SM1/1 or other commercial anti APO-1 antibodies
• Optimal dilutions should be determined by the individual laboratory for each application
• Applications Tested: Flow Cytometry
• Purity: >95%
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily
• FAS also exists in a soluble form that weight approximately 26kDa
• Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases
• The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis
• FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells
• At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described
• The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform
• The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer
• It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.