CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily
• CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts
• Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance
• 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain
• 15A7 does not induce apoptosis of Fas-expressing cells
• Applications Reported: This 15A7 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of mouse thymocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily
• FAS also exists in a soluble form that weight approximately 26kDa
• Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases
• The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis
• FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells
• At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described
• The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform
• The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer
• It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.