Invitrogen™ IP-10/CXCL10 Mouse Matched Antibody Pair

Description

• The Mouse IP-10 Matched Antibody Pair Kit comes with pre-matched antibody pairs,standards,sample dilutent and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IP-10
• Buffer reagents and microplates needed to complete the ELISA reaction are sold separately
• Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair
• A target-specific antibody is coated to the bottom of the wells of a microplate,which is an overnight process
• Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody
• A sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal
• The intensity of this signal is directly proportional to the concentration of target present in the original specimen
• CXCL10 ( C-X-C Motif Chemokine Ligand 10) is a 10 kDa protein and member of the CXC family that acts by binding to CXCR3
• Binding of CXCL10 to CXCR3 results in pleiotropic effects,including stimulation of monocytes,natural killer and T-cell migration,and modulation of adhesion molecule expression
• CXCL10 is a pro-inflammatory chemoattractant cytokine that attracts activated T lymphocytes
• CXCL10 is constitutively expressed at low levels in lymph organs,and the expression is highly induced by IFN-gamma and to some extent by IL12
• It is an important biomarker for diagnosis of many clinical conditions,and it plays an important role in host defense and inflammation
• CXCL10 exhibits an angiostatic and an antitumor role that is also involved in Th1 inflammatory diseases,Hashimoto′s thyroiditis,Graves′ disease and Type 1 diabetes mellitus.