Invitrogen™ IL-4 Porcine Matched Antibody Pair

Description

• The Porcine Interleukin 4 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of pig IL-4
• Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No
• CNB0011)
• The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate
• Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair
• A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process
• Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody
• A sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody
• A conjugated enzyme has been incorporated into the assay
• After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal
• The intensity of this signal is directly proportional to the concentration of target present in the original specimen
• The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells
• This cytokine is a ligand for interleukin 4 receptor
• The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13
• STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine
• This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13
• This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome
• Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.