Invitrogen™ Human IL-12 p40 Matched Antibody Pair

Description

• The Human Interleukin 12 p70 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-12 p70
• Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No
• CNB0011)
• The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate
• Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair
• A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process
• Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody
• A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal
• The intensity of this signal is directly proportional to the concentration of target present in the original specimen
• Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35)
• IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells
• IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells
• Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production
• These cytokines can in turn promote T-cell responses and macrophage activation
• Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system
• IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker
• The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4
• Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity
• Human and mouse IL-12 p40 share about 70% amino acid sequence homology
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