Invitrogen™ IL-6 Human Matched Antibody Pair

Description

• The Human IL-6 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-6
• Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No
• CNB0011)
• The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate
• Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair
• A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process
• Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody
• A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal
• The intensity of this signal is directly proportional to the concentration of target present in the original specimen
• Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells
• IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response
• IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells
• IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes
• In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes
• IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors
• IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma
• IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas
• Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha
• The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis
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