Invitrogen™ MMP3 Human Matched Antibody Pair

Description

• The Human MMP3 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MMP3
• Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No
• CNB0011)
• The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate
• Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair
• A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process
• Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody
• A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal
• The intensity of this signal is directly proportional to the concentration of target present in the original specimen
• MMP3 (stromelysin-1) belongs to the peptidase M10A subfamily
• It is secreted in its pro-protein form, and is activated by extracellular proteinase cleavage
• MMP3 is capable of binding to zinc and calcium ions, and has metallo-endopeptidase activity
• The active enzyme degrades collagen, gelatin, fibronectin, and laminin
• It is involved in normal cellular processes such as wound repair and tissue remodeling
• Mutations in the gene can result in coronary heart disease 6
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