MNAT1 Rabbit anti-Human, Polyclonal, Bioss

Description

• Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks)
• The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive
• Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires phosphorylation at Thr 160
• The enzyme responsible for phosphorylation of Thr 160 in Cdk2 and also Thr 161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit, a regulatory subunit and a subunit of unknown function
• The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7
• This previously undescribed protein, now termed Mat1, has been cloned as a protein that associates with the cyclin H-Cdk7 nuclear complex at all stages of the cell cycle
• Cyclin H-Cdk7-Mat1 complexes display kinase activity towards Cdk activation domains, and the carboxy terminus of RNA polymerase II
• Mat1 appears to constitute the first example of an assembly factor, essential for the formation of an active Cdk-cyclin complex.