beta GalactosidaseRabbit anti-Bacteria, Polyclonal, Rockland™

Description

• Store vial at 4°C prior to restoration
• For extended storage aliquot contents and freeze at -20°C or below
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Beta Galactosidase [E
• coli]
• Cross reactivity against Beta Galactosidase from other sources may occur but have not been specifically determined
• Beta Galactosidase is an exoglycosidase which hydrolyzes the beta-glycosidic bond formed between a galactose and its organic moiety
• Deficiencies in the protein in humans can result in galactosialidosis or Morquio B syndrome
• In E
• coli, the gene of beta-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low
• E
• coli beta Galactosidase is commonly used in molecular biology as a reporter marker to monitor gene expression
• Another popular use for beta Galactosidase is in blue/white screening to identify recombinant clones
• Beta Galactosidase can be split in two peptides, lacZalpha and LacZOmega, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme
• This property is exploited in many cloning vectors
• The presence or absence of an active beta Galactosidase may be detected through addition of artificial chromogenic substrates such as X-gal, fluorescent substrates such as Fluorescein di-beta-D-galactopyranoside (FDG), luminescent substrates and others
• Beta Galactosidase activity at pH 6 is an indicator of senescent cells not found in presenescent, quiescent or dividing cells.