50-199-0774
DYKDDDDK Mouse anti-Tag, Agarose, Clone: 29E4.G7, Rockland™
Manufacturer: Rockland Immunochemicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-199-0774-Each-of-1 | In Stock | ₹ 59,231.28 |
50-199-0774 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 59,231.28
GST (18%): ₹ 10,661.63
Total Price: ₹ 69,892.91
Antigen
DYKDDDDK
Classification
Monoclonal
Conjugate
Agarose
Gene Alias
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen
Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
Regulatory Status
RUO
Content And Storage
4°C
Form
Slurry
Target Molecule
Tag
Applications
Western Blot
Clone
29E4.G7
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species
Mouse
Quantity
1 mL
Primary or Secondary
Primary
Product Type
Antibody
Isotype
IgG2a κ
Description
- Store vial at 4°C prior to opening
- Anti-DYKDDDDK Affinity Gel is a purified mouse IgG2a monoclonal antibody coupled to activated agarose
- This product is intended for purification of proteins containing the FLAG™ epitope tag sequence
- Binding Specificity: Anti-DYKDDDDK Affinity Gel binds the FLAG™ epitope tag sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to the amino terminal, carboxy terminal or internal locations of targeted recombinant proteins expressed in transfected or transformed cells
- D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) is recommended for competitive elution to recover fusion protein (see protocol)
- DYKDDDDK Tag (FLAG™ tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology
- The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended
- A FLAG tag can be used in many different assays that require recognition by an antibody
- If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence
- Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis
- Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein
- The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function
- The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins
- FLAG is a trademark of Sigma-Aldrich Biotechnology LP
Compare Similar Items
Show Difference
Antigen: DYKDDDDK
Classification: Monoclonal
Conjugate: Agarose
Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen: Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
Regulatory Status: RUO
Content And Storage: 4°C
Form: Slurry
Target Molecule: Tag
Applications: Western Blot
Clone: 29E4.G7
Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 1 mL
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG2a κ
Antigen:
DYKDDDDK
Classification:
Monoclonal
Conjugate:
Agarose
Gene Alias:
DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
Immunogen:
Anti-DYKDDDDK Affinity Gel antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.
Regulatory Status:
RUO
Content And Storage:
4°C
Form:
Slurry
Target Molecule:
Tag
Applications:
Western Blot
Clone:
29E4.G7
Formulation:
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
1 mL
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG2a κ
Antigen: 6X His Tag
Classification: Monoclonal
Conjugate: Atto 550
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: Tag
Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Clone: 33D10.D2.G8
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 50 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
6X His Tag
Classification:
Monoclonal
Conjugate:
Atto 550
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Immunogen:
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
Tag
Applications:
ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Clone:
33D10.D2.G8
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
50 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Antigen: Phospho-AKT (Ser473)
Classification: Monoclonal
Conjugate: Atto 594
Gene Alias: Akt; Akt kinase; AKT serine/threonine kinase 1; AKT/PKB; AKT1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; AKT2; AKT3; CAKT; C-AKT; CG4006; CG4006 gene product from transcript CG4006-RE; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; DAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAkt1; DAKT1/PKB; Dmel\CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; kinase Akt1; l(3)04226; l(3)89Bq; MGC99656; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; p-Akt; Phospho-AKT; Phospho-PKB; PKB; PKB Akt; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; Rac; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; serine/threonine protein kinase; serine-threonine protein kinase; STK-2; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Immunogen: Akt phospho S473 ATTO594 Conjugated Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: __
Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Clone: 17F6.B11
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
Phospho-AKT (Ser473)
Classification:
Monoclonal
Conjugate:
Atto 594
Gene Alias:
Akt; Akt kinase; AKT serine/threonine kinase 1; AKT/PKB; AKT1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; AKT2; AKT3; CAKT; C-AKT; CG4006; CG4006 gene product from transcript CG4006-RE; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; DAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAkt1; DAKT1/PKB; Dmel\CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; kinase Akt1; l(3)04226; l(3)89Bq; MGC99656; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; p-Akt; Phospho-AKT; Phospho-PKB; PKB; PKB Akt; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; Rac; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; serine/threonine protein kinase; serine-threonine protein kinase; STK-2; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Immunogen:
Akt phospho S473 ATTO594 Conjugated Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
__
Applications:
ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Clone:
17F6.B11
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ
Antigen: 6X His Tag
Classification: Monoclonal
Conjugate: Atto 647N
Gene Alias: 6His tag; 6X His; His Tag; Histidine Tag
Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status: RUO
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: Lyophilized
Target Molecule: Tag
Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Clone: 33D10.D2.G8
Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 50 μg
Primary or Secondary: Primary
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
6X His Tag
Classification:
Monoclonal
Conjugate:
Atto 647N
Gene Alias:
6His tag; 6X His; His Tag; Histidine Tag
Immunogen:
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Regulatory Status:
RUO
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form:
Lyophilized
Target Molecule:
Tag
Applications:
ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Clone:
33D10.D2.G8
Formulation:
0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
50 μg
Primary or Secondary:
Primary
Product Type:
Antibody
Isotype:
IgG1 κ