IL-1 Receptor Antagonist Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use.  This is an IgG preparation of whole rabbit serum purified by DEAE chromatography
• In general, this antibody also detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra
• The antibody does not recognize human IL-1a, IL-1b or IL-1 receptor type I and type II
• This antibody is primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra) but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra)
• IcIL-1Ra is found in many cells as constitutively expressed protein, particularly epithelial cells and keratinocytes
• This antibody does not distinguish between sIL-1Ra and icIL-1Ra in ELISA formats or by immunohistochemistry
• When used in immunoblot analysis, it will recognize 17,000 MW IL-1Ra in supernatants released from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC)
• PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS
• However, in lysates of PBMC, icIL-1Ra is also found after 48-72 hours of culture
• In monocytic cells, the initial IL-1Ra transcript is for sIL-1Ra which can be found almost exclusively intracellularly
• The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family
• This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA)
• It is an important mediator involved in many cytokine induced immune and inflammatory responses
• This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.