Leptin Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-Leptin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
• The antibody will recognize leptin from mouse and human
• Reactivity with leptin from other sources is unknown
• Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function
• Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene
• Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body
• Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta
• Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells
• Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure
• Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors
• Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth
• Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension
• Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation
• Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.