ROBO-1 Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity purified antibody is directed against human ROBO-1 protein
• A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and dog sources based on 100% homology for the immunogen sequence
• Cross reactivity will occur with all isoforms of ROBO-1
• Cross reactivity with ROBO-1 homologues from other sources has not been determined
• Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior
• In the vertebrate spinal cord, the insect ventral nerve cord and in C
• elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively
• These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway
• One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals
• Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion
• Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross
• Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout
• Robo1 is also homologous to the C
• elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.