Phospho-Histone H2AvD (Ser137) Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Affinity purified Anti-Histone H2AvD pS137 Antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pS137 residue
• Reactivity occurs against Drosophila H2AvD pS137 protein and the antibody is specific for the phosphorylated form of the protein
• Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA
• A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence
• Reactivity against homologues from other sources is not known
• Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A
• H2AvD is vital for viability, but the exact function of variant histones H2A is not known
• H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4
• The octamer wraps approximately 146 bp of DNA
• HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only)
• The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur
• In mouse, H2AX ''knock out'' mice have an increased incidence of cancer.