Phospho-EGFR (Tyr1197)Rabbit anti-Human, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity-purified antibody is directed against the phosphorylated form of human EGFR protein at the pY1197 residue
• Reactivity occurs against human EGFR pY1197 protein and the antibody is specific for the phosphorylated form of the protein
• Reactivity with non-phosphorylated human EGFR is minimal by ELISA
• A BLAST analysis was used to suggest cross reactivity with phosphorylated EGFR from human, mouse and rat sources
• Reactivity of this antibody with EGFR from other species is unknown
• EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans
• EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4
• EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain
• Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase
• EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells
• Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway
• Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling
• Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found
• Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors
• Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.